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Cleavage of DNA by restriction endonucleases is a crucial component of any aspect of biological research. From genetics to protein analysis it is something you cannot avoid. Here's a generally accepted protocol in the Rao Lab.



In your tube add the following:

50 uL total volume

Digest for 1+ hr at 37C (or other temperature specified for your enzyme). Water baths are nice for this, but your incubator is fine too.