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Background

Colony PCR verification, or whole colony PCR, is a now standard method to verify the modification of plasmid and/or chromosomal DNA replacing older methods such as radio-labeled hybridization. The method works by using the cellular DNA (chromosomal and/or plasmid) of selected colonies as template and using specific primers to generate PCR products to verify the presence of DNA manipulations. The selection of appropriate primers is essential for this technique to work well and to provide useful information to the investigator. The primary advantage of this technique is speed; the investigator can screen 10's to sometimes 100's of colonies quickly and with adequate precision and accuracy.

You'll Need

Procedure