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Contents

Overview

The CRIM (Conditional-replication, integration, and modular) plasmid system is a series of plasmid vectors which use the R6K replicon for maintenance as an autonomous DNA element in pir+ hosts and contain an attP site for site-specific integration into the chromosome of non-pir+ hosts. Integration into non-pir+ hosts requires the expression of the corresponding lambdoid phage Int protein (ie integration into attBλ requires attPλ and Intλ). The procedure outlined here is directly adapted from the 2001 Haldimann and Wanner paper.

Materials

Method

Notes